Department of Mouse Genetics and Inflammation
Institute for Genetics/CECAD Cologne
Prof. Manolis Pasparakis
Together with the labs of
Prof. Dr. Hamid Kashkar
Institute for Molecular Immunology/University Hospital Cologne
Dr. Silvia von Karstedt
Research Group „Cell Death and Cancer Evolution”, CECAD Cologne
Summer Term 2023 (16.05. -14.07.2023)
Our module deals with the mechanisms regulating different pathways of regulated cell death (RCD) including apoptosis, necroptosis, pyroptosis and ferroptosis. You will get insights on important concepts concerning the functional implications of RCD in inflammation and immunity, as well as in the pathogenesis of inflammatory and degenerative diseases. In the lab you will perform molecular, biochemical and imaging methods used to detect and measure cell death as well as inflammatory responses. This will include eukaryotic cell culture and transfection, protein and DNA purification and analysis, gel electrophoresis (agarose and PAGE), western blot, immunofluorescence staining, immunohistochemistry (confocal and fluorescent microscopy), FACS and assays detecting different forms of cell death.
This course is mainly conducted by the lab of Prof. Manolis Pasparakis, together with the labs of Prof. Dr. Hamid Kashkar and Prof Dr. Silvia von Karstedt.
Click here for detailed course information
The course starts on Monday, 22nd of May at 9:15 am (Venue: Institute of Genetics at the University of Cologne Zülpicher Str. 47a, ground floor, room 0.46.).
An introductory meeting via ZOOM is planned for the 16th of May at 12:00. Here you will get the obligatory safety instruction. Additionally, you will get an overview on the dates and the structure of the course and the requirements specified therein. The ZOOM link for the safety instruction will be send to the participants via email.
Galluzzi, L. et al. Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018. Cell Death Differ. 1–56 (2018). doi:10.1038/s41418-017-0012-4
Broz P & Dixit VM (2016) Inflammasomes: mechanism of assembly, regulation and signalling. Nat Rev Immunol 16: 407–420
Hengartner MO (2000) The biochemistry of apoptosis. Nature 407: 770–776
Jorgensen I, Rayamajhi M & Miao EA (2017) Programmed cell death as a defence against infection. Nat Rev Immunol
Lamkanfi M & Dixit VM (2019) Manipulation of host cell death pathways during microbial infections. Cell Host Microbe. 8(1):44-54.
Oeckinghaus A & Ghosh S (2009) The NF-kappaB family of transcription factors and its regulation. Cold Spring Harb Perspect Biol 1: a000034
Pasparakis M & Vandenabeele P (2015) Necroptosis and its role in inflammation. Nature 517: 311–320
Vanden Berghe T, Linkermann A, Jouan-Lanhouet S, Walczak H & Vandenabeele P (2014) Regulated necrosis: the expanding network of non-apoptotic cell death pathways. Nat Rev Mol Cell Biol 15: 135–147
Varfolomeev E & Vucic D (2016) Intracellular regulation of TNF activity in health and disease. Cytokine
von Karstedt, S.*, Montinaro, A.*, and Walczak, H. (2017). Exploring the TRAILs less travelled: TRAIL in cancer biology and therapy. Nat. Rev. Cancer 17, 352–366.
Yang WS & Stockwell BR (2016) Ferroptosis: Death by Lipid Peroxidation. Trends Cell Biol 26: 165–176
Proneth, B., and Conrad, M. (2018). Ferroptosis and necroinflammation, a yet poorly explored link. Cell Death Differ. 1–11.